威九国际

图1 无参转录组分析流程图

      图1  SSR长度分布图                                     图2  DEG的MA-plot分布图

   图3  DEG的Volcano-plot分布图                        图4  转录因子家族分类

图5 差异基因GO功能富集结果

经典案例

红花转录组De novo研究发现与油质蛋白和黄酮合成相关的基因 PLoS ONE. 2012.

De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids. PLoS ONE, 2012, 7(2): e30987. doi:10.1371/journal.pone.0030987.

本文选用红花种子、叶、花瓣3个组织样品，提取Total RNA后进行转录组测序，对序列进行组装，基因差异表达分析以及GO、Pathway分析。组装得到了153,769个基因，其中8个基因与油质蛋白合成相关，23个基因与黄酮类化合物合成相关。

图1 GO分析结果

图2 黄酮素生物合成相关的Pathway分析

推荐文献

[1] De novo assembly and characterization of the transcriptome of the parasitic weed Cuscuta pentagona identifies genes associated with plant parasitism. Plant Physiology Preview. 2014, DOI:10.1104/pp.113.234864.

[2] Development of an expressed gene catalogue and molecular markers from the de novo assembly of short sequence reads of the lentil ( Lens culinaris Medik.) transcriptome. Plant Biotechnology Journal. 2012, doi: 10.1111/pbi.12082.

[3] De Novo Analysis of Transcriptome Dynamics in the Migratory Locust during the Development of Phase Traits. PLoS One. 2010, doi: 10.1371/journal.pone.0015633.